Last season the sides met twice in the league, with the away teams taking the spoils on both occasions, as Lancaster City beat Colne at Holt House before Colne repayed the favour, overturning City at Giant Axe.
Since then, Lancaster City have gone up and settled in a new league, whilst Colne have faced tough fixtures in the league below and occupy a mid-table birth.
The sides meet at 3pm on Saturday at the recently renamed Utility Renewals Stadium.
Admission is £8.
We want to see as many Dolly Blues there as possible, to help try and roar the side into the next round!
Updated 19:11 - 4 Sep 2017 by LCFC Media